Chromatography Lab Name_______________________________________________
Partners' Names
_______________________________________________
_______________________________________________
Chromatography
is a useful method for separating the substances in a mixture. In a mixture the
substances are not chemically combined, therefore, they can be separated.
Chromatography uses the fact that different substances dissolve at different
rates to separate those mixtures.
Purpose: To use chromatography to separate substances in a
mixture.
Show differences in physical properties in
a substance that make up a mixture.
Materials Filter paper Red,
green, blue and black markers. Pencil
Metric
ruler scissors Methanol water
Graduated
cylinder Beaker Paper towel
Procedure:
1. Cut four strips of filter paper at least 10 cm long.
2. Use a pencil to draw a line across the paper 1 cm from the bottom.
3. Use a pencil to label the paper as red, blue, green, black.
4. Make a
spot corresponding to the color on each of the papers, on the center of the
line.
5. From the center of the dot, measure up the center of the paper and
make a mark every centimeter.
6. Let the dot dry and darken the mark again.
7. Using the graduated cylinder, measure out 5 mL
of water, and pour into beaker.
8. Using the graduated cylinder, measure out 5 mL
of methanol and pour into the beaker.
9. Carefully stand all 4 paper strips in beaker, and crease edges to
keep them from falling
DO NOT ALLOW THE DOTS TO TOUCH THE LIQUID
10. Record observations until the solvent reaches
the edge of the beaker.
11. After the solvent has reached the edge of the beaker, remove the strips,
and put them on a piece of paper towel to dry. (CAUTION the dyes from the
marker can stain your hands and clothes. Do not touch the colored areas of the
paper.)
12.
Clean and dry beaker and graduated cylinder. Return all materials to where you
got them.
13.
Answer the questions.
14. Attach dried strips to sheet
Ink Color |
Observations |
Green |
|
Blue |
|
Red |
|
Black |
|
Questions:
1. Did a physical or
chemical change occur during this experiment? How do you know?
2. What colors of dye were
there in each color of ink? (i.e. What colors were
there in the green ink?)
3. Compare the order of the
colors on you strips. Is there a pattern to how the colors moved?
4. Predict what would happen
to
A. Orange ink
B. Purple ink
C. Yellow ink
Attach
the dried strips here